CHARACTERIZATION OF KEY PARAMETERS FOR BIOTECHNOLOGICAL LIGNOCELLULOSE CONVERSION ASSESSED BY FT-NIR SPECTROSCOPY. PART I: QUALITATIVE ANALYSIS OF PRETREATED STRAW

Chularat Krongtaew, Kurt Messner, Thomas Ters, Karin Fackler

Abstract


Wheat straw (Triticum aestivum L.) and oat straw (Avena sativa L.) were subjected to acid and alkaline pre-treatments partly in combination with hydrogen peroxide. The aim was to remove lignin and increase the accessibility of the polysaccharides to enzymatic digestion. Accessibility was evaluated by digestion with a cell wall degrading enzyme complex to yield reducing sugars that may serve as precursor substrates for biofuels or building block chemicals. Changes in lignin, hemicelluloses, as well as amorphous, semi-crystalline, and crystalline regions of cellulose moieties of pretreated straw were efficiently characterized by Fourier transform near-infrared (FT-NIR) reflectance spectroscopy. These alterations of the chemical structure of straw after different pre-treatment methods were powerfully differentiated by principal component analysis (PCA). Characteristics of the different samples owing to the different pre-treatment methods could be clustered from the PCA loadings spectra.

Keywords


Lignocellulose characterization; Wheat and oat straw; Mild acidic and alkaline peroxide pre-treatments; Fourier-transform near infrared spectroscopy (FT-NIR); Principal component analysis (PCA); Multivariate data analysis; Qualitative discriminant analysi

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