CONTROLLED ENZYMOLYSIS PREPARATION OF NANOCRYSTALLINE CELLULOSE FROM PRETREATED COTTON FIBERS

Xiaoquan Chen, Xueyan Deng, Wenhao Shen, Lingling Jiang

Abstract


Natural cotton fibers were pretreated with DMSO, NaOH, or ultrasonic waves and hydrolyzed by cellulase (Trichoderma vride G) to prepare nanocrystalline cellulose (NCC). The as-prepared samples were characterized by TEM, DLS, XRD, and FT-IR. The influences of the pretreatment on the yield and morphology of NCC were investigated. The alterations of the crystalline phase and chemical structure of NCC were also measured during the enzymolysis process. The experimental results proved that the pretreated cotton fibers could be hydrolyzed by the Trichoderma cellulase to prepare a nanosized strip (10 to 40 nm x 70 to 280 nm) and grainy (20 nm or 6 nm) crystalline cellulose, in which the different morphologies resulted from the different manners of pretreatment. The unaltered crystalline phase and chemical composition of NCC prepared by enzymolysis of pretreated cotton fibers were measured. The highest yield of NCC reached was 32.4%.

Keywords


Nanocrystalline cellulose; Cotton fibers; Cellulose; Pretreatment; Enzymolysis; Cellulase

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126