OPTIMIZATION OF PROTEIN PRODUCTION BY GEOTRICHUM CANDIDUM MIUG 2.15 BY CULTIVATION ON PAPER RESIDUES, USING RESPONSE SURFACE METHODOLOGY

Gigi Coman, Iuliana Leuştean, Luminita Georgescu, Gabriela Bahrim

Abstract


Response surface methodology (RSM) based on the 2^3 factorial central composite design (CCD) was used to optimize the biotechnological conditions for growth and protein production by a selected fungal strain Geotrichum candidum MIUG 2.15, by solid-state cultivation on a semisolid medium based on a mixture of paper residues, i.e. office paper, newspaper, and cardboard, mixed in a ratio of 1:1:1(w/w), supplemented with cheese whey waste and complex manure. Three independent variables, the solid:liquid ratio, the concentration of complex manure, and cultivation time, were evaluated to determine their correlative effect on biomass production and protein biosynthesis. The optimal conditions for obtaining a maximum protein yield of 9.53% w/w dry mass were the following: the complex manure concentration of 0.5%, the solid:liquid ratio of 1:5, and the growth time of 10 days.

Keywords


Geotrichum candidum; Protein production; Solid-state fermentation; Paper residues; Response surface methodology (RSM)

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