Optimizing the Extraction Yield of Polyprenols from Needles of Cunninghamia lanceolata (Lamb.) Hook using Response Surface Methodology and its Antioxidative Activities

Ping Jiang, Yang Zhang, Zongxing Shan, Qinghe Zheng

Abstract


An improved optimization method was used, combining a single-factor experiment and Response Surface Methodology to optimize the medium for the extraction yield of polyprenols from the needles of Cunninghamia lanceolata (Lamb.) Hook. A three-factor, three-level Box-Behnken design was used with extraction temperatures, extraction times, and liquid-solid ratio as independent variables to understand and optimize the extraction yield of polyprenols. A mathematical model with a high coefficient of determination was obtained and could be employed to optimize polyprenols extraction. From the optimized values of extraction temperature 71.4 ℃, extraction time 5.96 h, and liquid-solid ratio 9.3:1, the extraction yield of polyprenols was 1.22 ± 0.04% (N = 3), which agreed closely with the predicted value (1.27%). Besides, polyprenols were demonstrated to have a strong antioxidative ability in vitro. Scavenging percentages of superoxide radical and DPPH by polyprenols were up to 75.6% and 56.9%, respectively.

Keywords


Cunninghamia lanceolata (Lamb.) Hook; Polyprenols; Single-factor experiment; Response surface methodology; Antioxidative ability

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