Optimization of Nutrition Constituents for Xylanase Activity by Rhizopus stolonifer Under Solid-State Fermentation on Corncob

Zhicai Zhang, Jiashao Li, Fan Feng, Dan Liu, Qiaoxia Pang, Ming Li, Keping Chen


This study aims to optimize the medium for xylanase activity by a newly isolated strain of Rhizopus stolonifer JS-1008 (R. stolonifer JS-1008) under solid-state fermentation (SSF) on corncob. Four quantitative variables impacting the enzyme activity were selected through one-factor-at-a-time design. They were nitrogen source, initial moisture content (IMC), inorganic salt, and surface active agent. In addition, the interaction among these factors was further investigated by response surface methodology (RSM). Statistical analysis revealed that among these factors, IMC and urea significantly affected the xylanase activity. Our data indicate that the optimal medium contains (g/g dry corncob): urea, 0.15; ZnSO4, 0.022; Tween-80, 0.08; IMC, 3. Under the optimal condition, the xylanase activity reached its maximum, 13.90 U/g dry substrate (DS), on the 10th day of fermentation. This work provides a new potential strain to synthesize xylanase for biofuel production.


Xylanase; Rhizopus stolonifer; Solid-state fermentation; Corncob

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126