Covalent Immobilization of Lipase onto Citric Acid-Esterified Loofah Sponge

Jun Zhu, Jian Zhang, Qiumei Lai, Bo Jiang, Renmin Gong

Abstract


Loofah sponge was activated by thermochemical esterification between the carboxyl groups of citric acid and hydroxyl groups of cellulose to introduce free carboxyl groups, which were further reacted with lipase amino groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as the condensing reagent. This resulted in loofah sponge–immobilized lipase. Under the optimized immobilization conditions, the highest activity of immobilized lipase per gram of dry carrier was found to be 45.8 U/g. The immobilized lipase exhibited maximum activity at 40 °C, pH 8.0, while the optimal temperature and pH for the free lipase were 37 oC and 7.5, respectively. The immobilized lipase showed better thermal stability, storage stability, and reusability than free lipase.

Keywords


Lipase; Immobilization; Esterified loofah sponge; Carbodiimide; Amide linkage

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126