Biological Pretreatment under Non-sterile Conditions for Enzymatic Hydrolysis of Corn Stover

Lili Song, Hongbo Yu, Fuying Ma, Xiaoyu Zhang

Abstract


Pretreatment with white-rot fungi can effectively remove lignin and decompose the structure of biomass to enhance subsequent enzymatic hydrolysis. This study developed a novel fungal pretreatment of biomass, which was operated under non-sterile conditions. The white-rot fungus Irpex lacteus colonized stably on the non-sterile substrates and effectively degraded lignin. After non-sterile fungal pretreatment for 42 days, lignin was degraded by 43.8%. The maximum saccharification efficiency was 7-fold higher after enzymatic hydrolysis compared to that of raw corn stover. Furthermore, the production of ethanol from corn stover improved. During non-sterile biological pretreatment, several microorganisms coexisted with Irpex lacteus, and the microbial community generated abundant by-products that greatly improved the efficiency of enzymatic hydrolysis. Non-sterile fungal pretreatment presents a feasible and promising technology for the production of biofuels by integrating on-farm wet storage systems. Moreover, it provides a low-cost bioconversion process and a stable, secure, and environmentally friendly energy supply.

Keywords


White-rot fungi; Biological pretreatment; Non-sterile; Phospholipid fatty acid; By-products

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126