Enzymatic Saccharification and L-lactic Acid Fermentation of Corn Stover Pretreated with Liquid Hot Water by Rhizopus oryzae

Xiaomeng Kou, Ruifeng Yang, Jian Zhao, Jie Lu, Yanjun Liu


Corn stover was pretreated with liquid hot water (LHW) to enhance its enzymatic hydrolysis and L-lactic fermentation. The cellulose conversion rate and L-lactic acid concentration were used to evaluate LHW pretreatment performance. Results showed that the optimum conditions for the LHW pretreatment of corn stover are a reaction temperature of 190 °C for 20 min and a solid-to-liquid ratio of 1:10. The cellulase loading was 30 filter paper units per gram of oven-dried, water-insoluble solid. These conditions resulted in 92.3% conversion of cellulose to glucose. Sequential hydrolysis and fermentation using pretreated water-insoluble solid (WIS) produced an L-lactic acid concentration of 45 g/L. This study indicated that LHW pretreatment of corn stover is a suitable method for achieving high cellulose conversion and L-lactic acid concentration.


Enzymatic hydrolysis; L-lactic acid; Corn stover; Liquid hot water pretreatment

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