GC-MS Characterisation of Sapogenins from Sisal Waste and a Method to Isolate Pure Hecogenin

Jener David G. Santos, Alexsandro Branco

Abstract


Five steroidal sapogenins (tigogenin, neotigogenina, hecogenin, gloriogenin, and dehydrohecogenin) were characterised by gas chromatography coupled with mass spectrometry (GC-MS) from a hydrolysed extract of sisal waste. In addition, pure hecogenin, an important raw material for the pharmaceutical industry, was obtained from this waste by selective liquid-liquid extraction of saponins with only hecogenin as aglycone, followed by acid hydrolysis. The yield of pure hecogenin was 460 mg.Kg-1 of sisal waste.

Keywords


Agave sisalana; Sisal waste; Extraction; Steroids; Hecogenin

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