Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

Khairul Azly Zahan, Norhayati Pa'e, Ida Idayu Muhamad

Abstract


In this study, microbial cellulose production by Acetobacter xylinum 0416 using standardized liquid pineapple waste was carried out in a 4-L rotary disc reactor (RDR). The objective of this study was to optimize the process parameters for production of microbial cellulose in the RDR. The effects of the disc rotation speed (5 to 12 rpm), pH (3.5 to 7.5), fermentation period (3 to 6 days), and inoculum concentration (3 to 20% v/v) on the microbial cellulose production were investigated. The optimum microbial cellulose yield was obtained using 10% (v/v) of inoculum concentration, whereby four days’ duration gave the most productive yield. In addition, the highest production of microbial cellulose was obtained at a low disc rotation speed of 7 rpm and a pH of 5.0. Analysis of data performed a high coefficient of determination value (R2=0.875) represented by a mathematical model of optimized microbial cellulose production, Y = -200.437 + 7.180X1 + 69.869X2 + 4.369X3 + 1.867X4 – 0.512X12 – 6.766X22 – 0.585X32 – 0.079X42. From the results, it can be concluded that the foremost factors that affect the production of microbial cellulose in RDR were pH followed by inoculum concentration, disc rotation speed (rpm), and fermentation period.

Keywords


Microbial cellulose; Rotary disc reactor; Process parameters; Optimization; Response surface methodology; Pineapple waste; Acetobacter xylinum 0416

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126