Optimum Bioconversion Process of Wheat Straw to Glucose: Ethanol Pretreatment

Lanfeng Hui, Huangwei Xiong, Kunlun Du


The research aimed at optimizing the conditions of pretreatment of wheat straw and of enzymatic hydrolysis of the cellulose (ethanol pulp). The ethanol pretreatment process involving acid-catalytic and enzymatic hydrolysis were evaluated for bioconversion of wheat straw to glucose. The influence of the independent process variables on cellulose yields, cellulose contents, lignin contents, and the rate of lignin removed were analyzed over a broad range by the response surface methodology (RSM). The results of the factorial experiment showed that the significant external factors affecting acid-catalytic ethanol pretreatment of wheat straw were ethanol concentration, maximum temperature, acid dosage, and time at maximum temperature. By analyzing the response surface plots, the optimum process parameters for pretreatment were obtained as follows: ethanol concentration 65%, maximum temperature 180 ºC, acid dosage 1.2%, and time at maximum temperature 60 min. Pulps with residual klason lignin ranging from 9.27% to 13.56% (w/w) were prepared from wheat straw using the acid-catalytic ethanol pretreatment process and were evaluated for bioconversion using enzymatic hydrolysis of the cellulose fraction to glucose. The effects of temperature, pH value, time of enzymolysis, and cellulase dosage on the hydrolysis yield of cellulose were separately examined.


Response surface methodology; Wheat straw; Acid-catalytic ethanol pretreatment; Cellulase; Enzymatic hydrolysis

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126