Enhancement of Laccase Production from Pleurotus ostreatus PVCRSP-7 by altering the Nutritional Conditions using Response Surface Methodology

Potu Venkata Chiranjeevi, Moses Rajasekara Pandian, Sathish Thadikamala

Abstract


Submerged culture conditions for laccase production by Pleurotus ostreatus were optimized by response surface methodology (RSM). A total of six factors, carbon (glucose), nitrogen sources (urea and peptone), 2,5-xylidine (inducer), wheat bran (lignocellulosic material), and medium pH, were optimized. A total of 50 experiments were conducted, and the obtained data were modeled using a second-order polynomial. The optimized conditions show significant improvement in laccase expression, by approximately 3.5-fold (12,124 U/L).

Keywords


Lignolytic enzymes; Response surface methodology; Laccase; Optimization; Submerged fermentation; Bioprocess; Pleurotus ostreatus

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