Alkaline Xylanase Produced by Trichoderma reesei: Application in Waste Paper Pulp Bleaching

Qianli Ma, Rendang Yang


In this work, a facile, green approach to natural biomass bleaching is reported. It was found that Trichoderma reesei could produce alkaline xylanase and xylanase is highly active in alkaline environments. It has good environmental adaptability and could be used to reduce the use of bleaching chemicals for the pretreating process and degradation of hemicellulose so as to break the linkages of Lignin-carbohydrate complexes (LCCs). These particular properties benefit the pulp bleaching process and can improve the physical properties of the resulting paper. The optimum bleaching process conditions were as follows: dosage, 12 U/g; pH, 8 to 9; time, 60 minutes; and temperature, 70 °C. The xylanase can decrease the chemical oxygen demand (COD) of bleaching effluent by 41% as compared to the blank. Finally, after xylanase pretreatment, the whiteness, yellow index, tensile index, burst index, and elongation of the resulting paper were 54.8% ISO, 1.73, 33.93 N·m/g, 2.91 KPa·m2/g, and 2.91%, respectively.


Xylanase; Waste paper pulp; Bleach; Property

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126