Formation and Characterization of Pseudo-Lignin Microspheres during High-pressure Water Pretreatment

Maohua Yang, Jianmin Xing, Yuyang Liu, Yilan Liu, Tingzhen Mu, Jiemin Zhou


Microspheres were generated after subjecting corn stem rind to high-pressure water pretreatment. These microspheres can be divided into two categories according to their location: free microspheres and adsorbed microspheres. The formation and characterization of both microsphere types were studied. For the free microspheres, the maximum formation was reached at 180 °C; when the temperature was too high (200 °C) or too low (120 °C), no free microspheres were observed. As pretreatment temperature was varied, the morphologies of the free microspheres were different. For the adsorbed microspheres, their formation occurred over a wider temperature range, and their density increased with rising pretreatment temperature. Using Fourier transform infrared spectroscopy, many C=C and C=O bonds were identified on the surface of the free microspheres, indicating the presence of functional groups similar to those of lignin. Compared with untreated corn stem rind, the lignin signal of the residue surface was enhanced, which may be attributable to the increase in adsorbed microsphere quantity.


Pretreatment; Microsphere; Pseudo-lignin; Morphology

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126