Selection of Potential Fungi for Production of Cellulase-Poor Xylanase from Rice Straw

Nur Idayu Zahari, Umi Kalsom Md Shah, Ainun Zuriyati Mohamed Asa’ari, Rosfarizan Mohamad

Abstract


Recently, xylanase has attracted attention in the pulp and paper industry because of its bleach-boosting properties, which reduces chemical consumption during the bleaching process. Four fungi species, namely Penicillium oxalicum T3.3, Aspergillus niger ATCC 6275, Colletotrichum gloeosporioides, and Pycnoporus sanguineus, were studied to select which fungi can best produce cellulase-poor xylanase using rice straw as a substrate. The fungi were screened for xylanase production based on the clear zone formation on a malt extract agar (MEA) plate containing xylan. P. oxalicum T3.3 and A. niger ATCC 6275 showed greater clear zone formation on the agar plate than the other two studied fungi. These fungi were grown in a liquid medium containing rice straw as the substrate. P. oxalicum T3.3 showed the highest xylanase activity (65.89 U/mL) with lowest carboxymethyl cellulase (CMCase) (1.88 U/mL) and filter paperase (FPase) activity (0.16 U/mL) after four days of fermentation at 30 °C. P. oxalicum T3.3 produced higher xylanase activity when rice straw was used as a substrate compared to commercial xylan. Thus, cellulase-poor xylanase produced from P. oxalicum T3.3 has high potential for biobleaching application in the pulp and paper industry in terms of technical and biological performance and economical aspects.

Keywords


Xylanase; Penicillium oxalicum T3.3; Rice straw; Biobleaching; Pulp and paper

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126