Investigating Lignocellulose in Cornstalk Pretreated with Trametes pubescens Cui 7571 to Improve Enzymatic Saccharification

Xuejun Wu, Qi An, Yucheng Dai, Jing Si


This study investigated the degradation and enzymatic saccharification of cornstalk by white-rot and brown rot fungi. The fungal strains Trametes pubescens Cui 7571, Trametes velutina Dai 10149, and Antrodia wangii Cui 7568 were analyzed in solid-state fermentation cultures. Various extracellular enzyme activities were assessed to determine biochemical changes during the degradation process. Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis were used to determine the structural changes. A correlation analysis between the chemical composition of cornstalk, extracellular enzyme activities, and structural changes indicated that T. pubescens Cui 7571 broke down lignin efficiently and subsequently degraded cellulose, and hemicellulose digestion was not affected by the lignin barrier. Enzymatic hydrolysis demonstrated that the T. pubescens Cui 7571-pretreated samples increased cellulose and hemicellulose conversion in cornstalk. Overall, T. pubescens Cui 7571 displayed excellent performance as a biological pretreatment agent, and lignin played a significant role in the enzymatic saccharification of cornstalk.


White-rot fungi; Extracellular enzyme activity; Chemical composition; FTIR; XRD; Enzymatic hydrolysis

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126