Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme

Samiur Rashid Shah, Ukaegbu Chinonso Ishmael, Jaya Vejayan Palliah, Mohammed Fazli Farida Asras, Sharifah Suhaiza Binti Nik Wan Ahmad

Abstract


The saccharification of laccase-pretreated empty fruit bunch (EFB) was optimized in a lab-scale experiment using one-factor-at-a-time (OFAT) and response surface methodology (RSM). After pretreatment, the degree of delignification was checked by noting the weight loss (%) after pretreatment, and also by the quantity of total sugar produced after saccharification with cellulase enzyme. OFAT studies of saccharification of the pretreated EFB showed that the biomass was best saccharified using cellulase enzyme at the following conditions: enzyme concentration of 30 IU/g of EFB, substrate concentration of 5.0% w/v, 50 °C, saccharification time of 24 h, and pH 5. This combination exhibited the highest yield of total sugar (28% w/w). Although 29% w/w yield was achieved with an enzyme concentration of 40 IU/g of EFB, this increase in yield was not proportional to the increased enzyme concentration and, therefore, was considered insignificant. Statistical analysis of the combined effects of pH and temperature showed that pH had a more significant effect than the temperature on the saccharification process, based on a P < 0.05 significance level. The effect of pH on total sugar production was more significant than the temperature in both linear and quadratic functions. In sum, the saccharification of laccase-pretreated EFB should follow the optimized process conditions achieved in the current study.

Keywords


Empty fruit bunch; Laccase enzyme; Saccharification; Sugar

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126