Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue

Xin Li, Ximei Gu, Chenhuan Lai, Jia Ouyang, Qiang Yong


Production of fumaric acid from xylo-oligosaccharides manufacturing waste residue (XOR) by Rhizopus oryzae CICC 40351 was investigated in a simultaneous saccharification and fermentation (SSF) process. The fermentation conditions for SSF were optimized by an orthogonal design method to maximize the fumaric acid concentration. The highest fumaric acid concentration (12.54 g/L) was reached with a substrate loading of 5% (w/v) XOR in the SSF process at 38 °C. The fumaric acid concentration of the SSF process was 1.8 times greater than that of the separate hydrolysis and fermentation (SHF) process under the same conditions. In addition, the SSF process yielded 0.34 g/g of glucose, whereas the SHF process yielded only 0.20 g/g of glucose. The results indicated that the SSF process notably improved the production of fumaric acid from lignocellulose by R. oryzae.


Rhizopus oryzae; Fumaric acid; Simultaneous saccharification and fermentation; Lignocellulose

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126