Improved Itaconic Acid Production from Undetoxified Enzymatic Hydrolysate of Steam-Exploded Corn Stover using an Aspergillus terreus Mutant Generated by Atmospheric and Room Temperature Plasma

Xin Li, Ke Zheng, Chenhuan Lai, Jia Ouyang, Qiang Yong

Abstract


Itaconic acid production by Aspergillus terreus (A. terreus) was investigated using the undetoxified enzymatic hydrolysate of steam-exploded corn stover as the sole carbon source. The fermentation conditions for A. terreus were optimized based on glucose as the carbon source. Unfortunately, wild-type A. terreus did not grow in the undetoxified enzymatic hydrolysate. Therefore, atmospheric and room temperature plasma (ARTP) mutagenesis was applied to obtain A. terreus mutant AT-90. A. terreus mutant AT-90 grew and secreted itaconic acid in the undetoxified enzymatic hydrolysate. The highest itaconic acid concentration (19.30 g/L) with a yield of 36.01% was obtained from the undetoxified enzymatic hydrolysate of 10% (w/v) steam-exploded corn stover. This work demonstrated that the A. terreus mutant generated by ARTP efficiently improved itaconic acid production from lignocellulose-based carbon source.

Keywords


Itaconic acid; Aspergillus terreus; Atmospheric and room temperature plasma; Undetoxified enzymatic hydrolysate; Steam-exploded corn stover

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