Yield of Polyphenolic Substances Extracted From Spruce (Picea abies) Bark by Microwave-Assisted Extraction

Alexandra Sládková, Martina Benedeková, Ján Stopka, Igor Šurina, Aleš Ház, Petra Strižincová, Katarína Čižová, Andrea Škulcová, Zuzana Burčová, František Kreps, Jozef Šima, Michal Jablonský


Closed-system microwave-assisted extraction was applied to extract total phenolics from spruce (Picea abies) bark, using 96.6% ethanol as an extractant. The influence of particle size (0.3; 1.0; 2.5 mm), time (3 to 20 min), and temperature (60; 80; 100 °C) on polyphenol recovery was also studied. Higher extraction temperature and smaller particle size resulted in a higher yield of extracted polyphenols. However, the effect of extraction time on yield was more complicated. The effect of all three factors is tentatively explained.


Extraction; Microwave extraction; Spruce bark; Folin-Ciocalteu reagent

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