Chemical Modification of Laccase from Aspergillus oryzae and its Application in OCC Pulp

Yangmei Chen, Jinquan Wan, Qitang Wu, Yongwen Ma

Abstract


Laccase activity and stability were improved and modified through the interaction between laccase amino acid residues (e.g., amino groups (-NH2) and carboxyl groups (-COOH)) and the chemical reagents thiourea dioxide (TDO) and L-phenylalanine methyl ester hydrochloride (L-PME). Compared with the control sample, the activity of the laccase modified with these chemical reagents was increased by 209% and 50%, respectively. The stability of the laccase modified with L-PME increased by 56.9%. However, the laccase modified with TDO only improved slightly. It was clear that reagent L-PME was more efficacious than TDO. The paper formed with addition of L-PME-modified laccase exhibited better performance in terms of tensile strength, bursting strength, and tear strength, which increased by 13%, 10%, 9%, respectively, compared with the paper formed with unmodified laccase.

Keywords


Laccase; Modification; Thiourea dioxide; L- Phenylalanine methyl ester hydrochloride; OCC pulp

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126