Synergistic Fermentation of Camphor Leaves by Cellulolytic Fungus and Optimization of Reducing Sugar Production

Lei Zeng, Baiquan Zeng, Jienan Chen, Lin Zhang, Peng Zhan, Beiguo Hu, Yini Zhang


The degradation of lignocellulosic materials requires the synergy of multiple enzymes. Synergistic fermentation would be an alternative to a complete enzyme system and consequentially enhance enzymatic production. The present study used cellulolytic fungus of Penicillium decumbens, Aspergillus niger C112, and Trichoderma reesei RutC-30 as the starting strains, to screen for the best combination and proportion of synergistic fermentation. The experiments of different combinations and inoculum ratios for synergistic fermentation were performed, and Box-Behnken designs were adopted to optimize the fermentation conditions. The best combination of strains was confirmed as T. reesei RutC-30 and P. decumbens with an 8:2 inoculum ratio. The maximum sugar concentration was 1.48 g/L with CMCase (17.53 U/mL) and FPase (4.78 U/mL) in this situation. Response surface methodology revealed the optimized parameters as a substrate concentration of 5.93% at 32.12 °C, and a fermentation time of 5.85 d with a predicted sugar concentration of 2.91 g/L. Under these conditions, a maximum sugar production of 2.96 g/L was achieved in verification experiments.


Camphor leaves; Synergistic fermentation; Cellulose conversion; Cellulase; Fermentation conditions optimization

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