Kinetics of Cellulase Saccharification of Corn Stover after Pretreatment by Lignin Peroxidase and H2O2

Zhi C. Zhang, Jin H. Li, Feng Wang

Abstract


The kinetics of cellulase saccharification of corn stover (CS) after pretreatment by lignin peroxidase (LiP) and H2O2 was modeled in this work. The Impeded Michaelis model was applied in fitting all experimental data. The model gave the initial activity and accessibility of the enzyme on the substrate (Kobs,0) and the gradual loss of enzyme activity (Ki). The maximum Ytrs (55.56%) was obtained at pH 4.7, 48.6 °C, a 1.5% cellulase, and 12.4:1 water-to-material ratio. The binary quadratic model provide a good fit of the data on Ytrs and of the model parameters Kobs,0 and Ki. The results showed that Ytrs was positively correlated with Kobs,0 and negatively correlated with Ki. This study laid a foundation for improving the cellulase saccharification efficiency of lignocellulosic biomass after pretreatment by H2O2 and LiP.

Keywords


Kinetics of Cellulase Saccharification of Corn Stover after Pretreatment by Lignin Peroxidase and H2O2

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