Butyric Acid Fermentation by Clostridium tyrobutyricum in Sugar Mixtures and Corncob Hydrolysate Containing Arabinose

Tianren Chen, Ling Zhang, Guanghong Luo, Wenqiao Yuan


The objective of this study was to understand arabinose effects on butyric acid fermentation by Clostridium tyrobutyricum in sugar-supplemented media and biomass hydrolysate. Compared to glucose and xylose, the conversion of arabinose to butyric acid was less efficient with a longer lag phase (4 d) and fermentation period (6 d), and a lower butyric acid yield of 0.26 g/g and 0.15 g/g in 5 g/L and 10 g/L of arabinose media, respectively. However, the addition of 2 g/L or 5 g/L of arabinose to the baseline medium that contained glucose (15 g/L) and xylose (15 g/L) enhanced butyric acid synthesis, resulting in 16.1 g/L and 20.3 g/L of butyric acid, respectively. Further increase in arabinose to 10 g/L in the medium of 10 g/L of glucose and 10 g/L of xylose showed inhibitory effects on C. tyrobutyricum, which suggested that high concentrations of arabinose (> 10 g/L) were not desirable. Dilute-acid pretreated corncobs that contained xylose (19 g/L) and a small amount of arabinose (2 g/L) were a feasible substrate for butyric acid production by C. tyrobutyricum, and resulted in 10.6 g/L butyric acid, which was slightly lower than in the mimic medium (11.3 g/L).


Clostridium tyrobutyricum; Butyric acid; Corncob; Xylose; Lignocellulosic biomass; Fermentation; Arabinose

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126