Production of Butanol from Acetyl Chloride-treated Deoiled Rice Bran by Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Abdualati Ibrahim Al-Tabib, Mohd Sahaid Kalil


Butanol was produced from pretreated deoiled rice bran (DRB) in a batch culture of Clostridium acetobutylicum YM1. The DRB was pretreated by acetyl chloride to produce fermentable sugars prior to butanol fermentation. Pretreatment of DRB using 1% acetyl chloride (AC-DRB) resulted in sufficient fermentable sugars (30.88 g/L), which was comparable to that produced by using 1% sulfuric acid (33.5 g/L). Pretreated AC-DRB contained 18.08 g/L glucose, 9.95 g/L xylose, and 2.86 g/L cellobiose. Detoxification of AC-DRB was performed to remove the fermentation inhibitors, such as furfural, 5-hydroxymethyl furfural (HMF), acetic acid, formic acid, and levulinic acid with the removal efficiencies of 92.98%, 98.82%, 51.53%, 38.72%, and 96.21%, respectively, using charcoal. The detoxification with charcoal was more efficient compared to that with XAD-4 resin. Acetone-butanol-ethanol (ABE) fermentation of detoxified AC-DRB (with 1% AC) by XAD-4 produced 5.64 g/L butanol, while detoxification with charcoal of AC-DRB (with 1% AC) produced 6.48 g/L butanol. In detoxified AC-DRB with charcoal, the maximum butanol and ABE yield of 6.48 g/L and 11.82 g/L, respectively, were achieved. This study is the first reported treatment of biomass using acetyl chloride, which was used as a pretreatment method for successful butanol production.


Butanol; Deoiled rice bran; Acetyl chloride Pretreatment; Clostridium acetobutylicum YM1

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126