Cellulose Accessibility and Zeta potentials of Sugarcane Bagasse Pretreated by Green Liquor and Ethanol for High Hydrolysis Efficiency

Wenwen Xue, Fuhou Lei, Pengfei Li, Jianxin Jiang

Abstract


Green liquor (GL) combined with ethanol (GL-ethanol) was selected to pretreat sugarcane bagasse (SCB). The results showed that the maximum lignin removal of 85.2% was achieved at 160 °C and a GL loading of 1.5 mL/g-dry substrate. The glucose yield of pretreated SCB increased with increased pretreatment temperature, and the maximum glucose yield of 97.7% was reached from SCB pretreated at 160 °C. Simons’ stain (SS) showed that the glucose yield was affected by cellulose accessibility instead of lignin content when lignin removal was > 70%. The cellulase adsorption isotherm fitted by the Langmuir model showed that the strength of interaction between the cellulase and substrate of GL-ethanol-100/1.5 (100 °C, 1.5 mL GL/g-dry substrate) was declining with increased pH. The adsorption was pH-dependent, and negatively controlled by the pH value. Electrostatic interactions can account for the pH-dependency of cellulase adsorption.

Keywords


Sugarcane bagasse; Green liquor-ethanol pretreatment; Enzymatic hydrolysis; Simons’ stain; Adsorption isotherm

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