Synthesizing Magnetic Support for Laccase Immobilization for the Purification of Pre-hydrolysis Liquor

Qiang Wang, Tianzhong Yuan, Shanshan Liu, Xin Fu, Guihua Yang, Jiachuan Chen, Wenhai Li, Rendang Yang, Lucian Lucia


Laccase immobilization is a promising method for the purification of pre-hydrolysis liquor (PHL) for large-scale applications based on recyclability and reusability. In this work, magnetic supports made of Fe3O4/SiO2 were synthesized by a sol-gel method and coated with silica. Then, glutaraldehyde was used as a linker between the support and laccase for immobilization. The immobilized laccase was evaluated by purifying the PHL from a kraft-based dissolving pulp production process. The results showed that the optimal immobilization conditions were a molar TEOS/Fe3O4 ratio of 0.6, glutaraldehyde concentration of 6%, laccase concentration of 0.4 mL/mg, and time of 3 h, which led to 71% laccase immobilization. The concentration of total sugar in the PHL increased from 71% to 75%, while the lignin content decreased from 15% to 10% after the immobilized laccase treatment. The separation advantage of the magnetic support enhanced the utility of the laccase.


Magnetic support; Laccase; Immobilization; Recovery

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Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126