Enzymatic Potential of Native Fungal Strains of Agave Residues

Maria Gorety Contreras-Hernández, Elva T. Aréchiga-Carvajal, Martha Rocio Moreno-Jiménez, Silvia Marina González-Herrera, Javier López-Miranda, Lilia Arely Prado-Barrágan, Olga Miriam Rutiaga-Quiñones

Abstract


Twelve strains of fungi from residues of Agave durangensis were isolated and identified by molecular techniques for evaluation of their hydrolytic enzyme production capability. A proportion (50%) of the fungal strains belonged to the Aspergillus genus and the other strains used belonged to Alternaria, Neurospora crassa, Mucor sp, Rhizopus sp., Botryosphaeria sp., and Scytalidium sp. The isolated strains were evaluated for their potential to produce extracellular enzymes using different substrates (cellulose, xylan, inulin, Agave fructans, starch, and tannic acid). It was observed that most of the tested strains were capable of simultaneously secreting cellulases, xylanases, inulinases, fructanases, and laccases. Botryosphaeria sp. ITD-G6 was selected for its evaluation in the production of inulinase, using different substrates. Showing high inulinase activities (5.22 U / mL for Agave waste, 4.37 U/ mL for inulin and 5.00 U / mL for Agave fructans).

Keywords


Inulinase; Fructanase; Molecular identification; Agave durangensis leaves; Botryosphaeria

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