Selective Biodegradation of Grape Pomace Tannins by Aspergillus niger and Application in Wood Adhesive

Ping Lan, Nicolas Brosse, Ju Qing Cui, Hai Yan Mao, Rui Yang


The selective biodegradation of grape pomace tannins was studied using Aspergillus niger strains. The conditions were optimized in terms of monomeric catechin production. The optimal degradation conditions were: 10.0 g·L-1 of tannins in the induced medium, pH 6.5, and cultured on a shaking table at a rate of 120 r·min-1 at 28 °C for 36 h. The biodegraded tannins were used to partially substitute for phenol-formaldehyde in adhesive formulations. The biodegradation enabled the production of adhesives and wood panels with better properties (lower resin viscosity, higher internal bond strength, and lower thickness swelling). A particleboard in which the resin contained 60% biodegraded grape tannins displayed good performances (IBdry = 0.46 MPa, IBwet = 0.15 MPa) and passed the standard specifications for dry and wet conditions.


Grape pomace tannins; Selective biodegradation; Catechin; Aspergillus niger; Phenol-formaldehyde adhesive

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