Activation of Cellulosic Ethanol Lignin by Laccase and its Application as Plywood Adhesive

Wenbiao Xu, Xiangyu Li, Junyou Shi


To reuse biorefinery waste, cellulose ethanol lignin was treated with a laccase system and used as an adhesive for plywood panel preparation. The effects of the amount of laccase, the pH of the reaction system, the reaction temperature, and the reaction time on the bonding strength were studied. The reaction characteristics of the lignin were analyzed by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and nuclear magnetic resonance (NMR). The results showed that the amount of laccase and the pH value of the reaction system had a significant effect on the bonding strength, and the addition of Tween-80 and polymeric diphenylmethane diisocyanate (PMDI) could improve the wet strength of the cemented system. FT-IR indicated that the lignin had been etherified and NMR analysis showed the partial ether bond in the β-O-4 structure was cleaved so that the lignin fragment was involved in the gluing of the small molecules. Microscopically speaking, the SEM analysis did not observe the activation of lignin adhesives infiltrating the wood substrate, indicating a weak mechanical gluing.


Laccase-mediator system; Biorefinery residue; Wood adhesives; Lignin; Biomaterials

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Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022,; Lucian A. Lucia, (919) 515-7707, URLs:; ISSN: 1930-2126