Determination of Lignin Monomer Contents in Rice Straw Using Visible and Near-infrared Reflectance Spectroscopy

Zhen Hu, Guifen Zhang, Yuanyuan Chen, Youmei Wang, Yuqing He, Liangcai Peng, Lingqiang Wang

Abstract


Genetic modification of plant lignin composition is an important strategy for improving biomass enzymatic digestibility without sacrificing the normal growth of the plant. However, the absence of fast and convenient methods for rapid determination of lignin composition has impeded corresponding research. Near-infrared reflectance spectroscopy (NIRS) analysis has potential as a solution for this dilemma, while the NIRS measurement for expediently assaying lignin composition in rice straws is still lacking. In this study, visible and near-infrared reflectance spectroscopy (VIS/NIRS) and modified partial least squares (MPLS) method were combined to develop calibration models for predicting the lignin monomer contents in a diverse rice population. Four optimal equations for predicting the content of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin units and their total amount (H + S + G) were generated with acceptable determination coefficients for calibration (0.85 to 0.93), cross-validation (0.75 to 0.88), and external validation (0.82 to 0.88), and the ratio performance deviation (RPD, 2 to 3.01). This study was the first to demonstrate that VIS/NIRS could give a sufficiently accurate prediction of lignin monomer contents in rice and could be applied for rapid assessments of large-scale rice straw samples.

Keywords


Visible and near-infrared reflectance spectroscopy (VIS/NIRS); Calibration models; Lignin monomer; Rice

Full Text: PDF

Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucian.lucia@gmail.com URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126