Enhancing the Ethanol Yield from Salix Using a Clostridium thermocellum and Thermoanaerobacterium thermosaccharolyticum Co-Culture System

Jian Pang, Min Hao, Yali Shi, Yongli Li, Mingda Zhu, Jianhua Hu, Jianguo Liu, Qiancheng Zhang, Zhanying Liu


A strategic method that utilizes the co-culture of Clostridium thermocellum ATCC 27405 and Thermoanaerobacterium thermosaccharolyticum DSM 571 was developed to improve the ethanol yield from the thermophilic fermentation of Salix. The co-culture conditions of the two strains were optimized using single factor and response surface experiments to enhance the ethanol yield. An even higher ethanol yield was obtained under the optimum co-culture conditions in fermenter tanks than what was observed in pre-experiments in serum bottles. The maximal ethanol concentration and yield were 0.2 g/L and 11.1%, respectively, and with a 26.4% cellulose degradation ratio and 13.8% hemicellulose degradation ratio when the pH was kept stable at 7.0 in fermenter tanks.


Clostridium thermocellum ATCC 27405; Thermoanaerobacterium thermosaccharolyticum DSM 571; Co-culture; Salix; Fermenter tank; Ethanol

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