Improving Biorefinery Sustainability and Profitability by Cultivating Aquatic Plants on Ozonized Distillery Effluents


  • Piotr Dziugan Lodz University of Technology
  • Zdzisława Romanowska-Duda University of Lodz
  • Krzysztof Piotrowski University of Lodz
  • Weronika Cieciura-Włoch Lodz University of Technology
  • Hubert Antolak Lodz University of Technology
  • Krzysztof Śmigielski Lodz University of Technology
  • Michał Binczarski Lodz University of Technology
  • Izabela Witońska Lodz University of Technology
  • Jaroslaw Domanski Lodz University of Technology


Biorefinery, Post-fermentation effluent, Ozone treatment, Lemnaceae


Industrial production of biogas offers a way to manage distillery leachate. The waste is usually subjected to anaerobic digestion for producing biogas. However, the effluent from anaerobic processes has high chemical oxygen demand (COD) and is harmful to the environment. An effective method of lowering COD is ozonation. Effluent from biogas plants after ozonation has the potential for use in breeding grounds for plants of the Lemnaceae family. Thus, they can provide a valuable additional source of biomass for the production of bioethanol. Lemna minor L. and Spirodela polyrhiza cultures were grown in media with the addition of 2.5% PFE, which had been treated by ozonation for between 6 and 50 min. Using ozonated effluent was an effective cultivation technique in all variants. The analyzed parameters were plant growth, chlorophyll index, fresh plant weight and photosynthetic traits (net photosynthesis, stomatal conductance, transpiration and concentration of intercellular CO2). The best growth of Lemna minor L. was observed in the media with PFE treated for 12 min. Similar effects were obtained for S. polyrhiza, with ozone treatment for 12 and 25 min. The results show the potential of using ozone-treated post-fermentation leachate as a supplement in culture media.






Research Article or Brief Communication