Fed-batch Enzymatic Hydrolysis of Steam-exploded Sugarcane Bagasse


  • Marlon Gualberto Ganter de Moura Federal University of Paraná
  • Thiago Alessandre da Silva Federal University of Paraná
  • Arion Zandoná Filho Federal University of Paraná
  • Marcos Lúcio Corazza Federal University of Paraná
  • Luiz Pereira Ramos Federal University of Paraná (UFPR) https://orcid.org/0000-0002-4349-4990


Sugarcane bagasse, Steam explosion (SE), Fed-batch enzymatic hydrolysis, Kinetic modeling


Statistical design and mathematical modeling were used to investigate the fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse (195 °C, 7.5 min). First, a Box-Behnken experimental design was used to evaluate the effect of enzyme loading (8, 24, and 40 FPU g-1 glucans of Cellic CTec3®), stirring speed (100, 150, and 200 rpm), and substrate total solids (5, 12.5, and 20 wt%) on the release of glucose equivalents (GlcEq, mostly glucose) after hydrolysis for 48 h in batch mode. A simplified kinetic model was used to fit the experimental data, in which specific activities in Cellic CTec3 were not differentiated, enzyme adsorption was ignored, and end-product inhibition was only attributed to glucose accumulation. The adjusted kinetic model was used to predict the effects of substrate and enzyme intermittent feedings in fed-batch hydrolysis experiments. Compared with batch experiments at 20 wt%, the proposed fed-batch procedure was able to increase GlcEq productivity by nearly 68% using the same enzyme loading, producing substrate hydrolysates containing 91.8 g L-1 GlcEq.







Research Article or Brief Communication