Pratima Bajpai, Aradhna Anand, Nirmal Sharma, Shree P. Mishra, Pramod K. Bajpai, Dominique Lachenal


The delignification efficiency of different laccase enzymes was examined on the eucalyptus Kraft pulp. Laccase enzyme from Trametes versicolor showing the highest delignification was selected and used in the elemental chlorine free bleaching sequence. An appreciable reduction in chlorine dioxide consumption was also obtained. Further reduction in chlorine dioxide consumption was obtained when the same laccase treated pulp was subjected to an acid treatment after the extraction stage followed by DEpD sequence. Elemental chlorine free bleaching was also performed using the xylanase-laccase treated pulp. Xylanase treatment was incorporated to the laccase mediator system in the elemental chlorine free bleaching both sequentially and simultaneously. The bleaching sequence DEpD was followed and in both the cases, reduction in chlorine dioxide consumption was more in comparison to control. Chlorine dioxide consumption reduced further when xylanase-laccase treated pulp was given an additional acid treatment. The final pulp properties of the treated pulps were comparable to the control pulp.


Papermaking; delignification; laccase; laccase mediator; xylanase; ECF bleaching; chlorine dioxide

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