Recycling Cellulase from Enzymatic Hydrolyzate of Laser-Pretreated Corn Stover by UF Membrane

Shuang-Qi Tian, Xin-Wei Wang, Ren-Yong Zhao, Sen Ma


The ultrafiltration membrane reactor, utilizing a membrane module with a suitable molecular weight alleyway, retains the larger cellulase components. Smaller molecules, such as the fermentable reducing sugars and water, pass through the membrane. The purpose of this work was to investigate the capability of recycling cellulase in the UF membrane. PS30 hollow fiber membrane, an ultrafiltration method using internal pressure, was found to be an ideal membrane separation device, allowing re-use of the enzyme. A Box-Behnken experimental design (BBD) established the following optimum pretreatment parameters: operation pressure at 1.73 bar, temperature at 36.38 °C, and a pH of 5.92. Under these conditions, the model predicted a membrane flux yield of 2.3174 L/(m2•h). The rejection rate of the UF membrane was over 95%.


Ultrafiltration; Cellulase; Hydrolyzate; Recycling

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