A Combined Process for Production of Fumaric Acid and Xylooligosaccharides from Corncob

Xin Li, Lei Yang, Ximei Gu, Chenhuan Lai, Caoxing Huang, Qiang Yong


Production of fumaric acid and xylooligosaccharides from corncob was investigated using a combined process. Corncob was fractionated into a cellulose-rich fraction and a hemicellulose-rich fraction by an alkali pretreatment. The cellulose-rich fraction was converted into fumaric acid by Rhizopus oryzae in fed-batch simultaneous saccharification and fermentation (SSF). Maximal fumaric acid concentration reached 35.22 g/L at a final 15% (w/v) solid loading in the fed-batch SSF. The hemicellulose-rich fraction was converted into xylooligosaccharides (XOSs) by endo-β-1,4-xylanase. The yield of XOSs was 62.35% after 24 h of xylanase hydrolysis. Xylobiose, xylotriose, and xylotetraose were the three major components in the XOSs. A mass balance analysis demonstrated that 100.6 g of fumaric acid and 148.1 g of XOSs were produced from 1000 g of dry corncob matter. The production of fumaric acid and XOSs by the combined process could make the utilization of corncob more efficient and more promising.


Corncob; Fumaric acid; Xylooligosaccharides; Fed-batch simultaneous saccharification; Fermentation; Rhizopus oryzae; Enzymatic hydrolysis; Xylanase

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