Analysis of the Differences in the Microbial Community and Structure of Calcified ONP Granular Sludge and Bagasse Granular Sludge

Zhiwei Wang, Cancan Zhu, Min Yi, Shuangfei Wang, Zhenzhen Yang, Qiming Feng


Anaerobic biological technology has been widely used in the treatment of high-concentration organic wastewater such as old newspaper (ONP) pulping wastewater and bagasse spray washing wastewater before pulping. However, due to the high calcium content and complex chemical properties of the ONP pulping wastewater, granular sludge calcification occurs during the anaerobic treatment, which has negative effects. In contrast, calcification does not occur in the bagasse spray washing wastewater. Therefore, a comparative analysis of the biological flora and structure of the granular sludge for these two types of wastewater can provide theoretical and data support for revealing the cause of calcification of granular sludge. The results indicate a considerable difference between the anaerobic granular sludge for the treatment of bagasse spraying wastewater (B-GS) and the treatment of ONP pulping wastewater (P-GS). The microorganisms in the B-GS were mainly Bactericides (25.4%), Proteobacteria (20.2%), Hyd24-12 (14.4%), Chloroflexi (10.6%), and Firmicutes (8.9%). The microorganisms in the P-GS were mainly Bacteroidetes (20.4%), Chloroflexi (19.5%), Proteobacteria (19.3%), Firmicutes (6.2%), Spirochaetae (4.8%), Actinobacteria (4.4%), and Lentisphaerae (4.3%). Methanomassiliicoccus were detected only in the P-GS. The methanogens had a higher relative abundance in the P-GS (50%), and only a small fraction (10%) of methanogens was detected in the B-GS.


High-throughput sequencing; Microbial community; Microbial diversity

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