Production of Bioethanol from Hybrid Cassava Pulp and Peel using Microbial and Acid Hydrolysis

Vincent E. Efeovbokhan, Louis Egwari, Edith E. Alagbe, James T. Adeyemi, Olugbenga S. Taiwo


Agricultural wastes are potential sources for the commercial production of biofuels because of their availability and low market price. In the present study, the viability of producing bioethanol from three varieties of cassava pulp and peel (CPP) was studied. Acid hydrolysis was performed by dispersing 20% w/v CPP in 100 mL of hydrochloric acid. Biological hydrolysis was performed by inoculating gelatinized CPP paste with Aspergillus niger. A set of un-gelatinized control samples was used to investigate the effect of heat pretreatment on the reducing sugar yield. The hydrolyzed samples were fermented with Saccharomyces cerevisiae, and the ethanol yield was determined. The reducing sugar yield was 110.7 g/L, 100.4 g/L, and 96.7 g/L from acid hydrolysis of three cassava varieties, while a yield of 98.9 g/L was obtained from cassava peel at 0.7 M and 50 min. The gelatinized pulp from the samples hydrolyzed with A. niger consistently produced more reducing sugar than the control samples. The highest ethanol yields were 54.8% and 33.1% obtained, respectively, from a heat-pretreated variety and cassava peel. Results from the conversion of cassava peel readily bring to light a more useful way of managing cassava wastes in the environment.


Cassava pulp; Cassava peels; Hydrolysis; Fermentation; Ethanol; Glucose

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