Identification of Antimicrobial Compounds in Different Hydrophilic Larch Bark Extracts

Kerstin Wagner, Caroline Roth, Stefan Willför, Maurizio Musso, Alexander Petutschnigg, Gertie Janneke Oostingh, Thomas Schnabel

Abstract


Larch bark is an undervalued material, but it has unique and valuable characteristics and compounds. The objective of this study was to extract and characterize molecular compounds of bark materials from different larch trees and to test these for their antimicrobial properties. The extractions were performed using methanol or water. The obtained extracts were analysed by gas chromatography coupled to mass spectrometry (GC-MS). Antimicrobial properties also were determined using different microbial strains, for example, Staphylococcus aureus. The GC-MS analysis showed that long chain alcohols, fatty acids, and polyphenols were present in the extracts. According to the results of the agar diffusion tests, only the methanol extract of larch bark had an inhibitory effect on the growth of Staphylococcus aureus. Two compounds of flavonoids and stilbenoids were shown to affect the microbial activity of the larch bark. Therefore, larch bark can be used for the extraction of compounds with specific anti-microbial properties.

Keywords


Agar-diffusion test; Gas chromatography – mass spectrometry; Solid-liquid extraction; Larch bark

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