Enhanced Production of Lipid as Biofuel Feedstock from the Marine Diatom Nitzschia sp. by Optimizing Cultural Conditions

Baala Harini Anandapadmanaban, Renganathan Rajkumar, Mohd Sobri Takriff

Abstract


Microalgae were isolated, identified, and cultivated for enhanced biomass production and lipid accumulation. A marine microalgae was isolated from coastal rock pools of Tuticorin, Tamil Nadu, and identified as Nitzschia sp. RRSE2 upon microscopic examination and molecular sequence analysis. The experimental results showed that the maximum growth, biomass, and lipid content were obtained at pH 8 using the F/2 medium. These parameters revealed a notable difference when NaCl was added at 3% concentration. Meanwhile, the nutrients NaNO3 (18.75 mgL-1) and NaH2PO4 (3.48 mgL-1) were shown to be suitable nitrogen and phosphorus sources, respectively, for the production of lipids. On day 14, the maximum lipid concentration of 77.5 mgL-1 was produced using optimized culture conditions. Additionally, the maximum number of 17×105 cells mL-1 and the biomass concentration of 0.69 gL-1 were achieved on this same day. Finally, the fatty acid composition of the algal lipid was analyzed by gas chromatography/mass spectrometry (GC/MS) analysis.

Keywords


Isolation; Identification; Nitzschia; Biomass; Lipid

Full Text:

PDF


Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucia-bioresources@ncsu.edu URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126