Effects of Pine (Pinus densiflora) Sawdust on Cordycepin Yield from Medicinal Fungus Cordyceps militaris in Submerged Culture

Si Young Ha, Ji Young Jung, Jai Hyun Park, Chan Yeol Yu, Jae-Hyeong Park, Jae-Kyung Yang


Cordycepin (3′-deoxyadenosine) is a nucleoside analog that exhibits a broad spectrum of biological activity. The effects of different tree sawdust on cordycepin as bioactive substances for mycelium growth were investigated. Pine sawdust was essential for increasing cordycepin content. Furthermore, a 1% NaOH-pretreated pine sawdust produced the highest cordycepin yield. The cordycepin yield of mycelium in submerged culture was significantly increased when the particle size was 100-mesh and the weight was 20 g/L of 1% NaOH-pretreated pine sawdust, with an increase of up to 38% compared to the control (only sabouraud dextrose broth (SDB)). The results demonstrated the effects of different tree sawdust on the biosynthesis of cordycepin as bioactive substances and that replacing traditional medium (SDB medium) with 1% NaOH-pretreated pine sawdust can increase the yield of cordycepin. After optimization of cordycepin production from Cordyceps militaris cultivated in medium containing 1% NaOH-pretreated pine sawdust using RSM (response surface methodology) BBD (Box-Behnken design) in its canonical form, the optimum combination was: particle size, 113.7-mesh; input weight, 11.9 g/L; and incubation time, 67.8 h. The model predicted a maximum yield of 922.6 μg/mL for cordycepin.


Cordyceps militaris; Cordycepin; Wood sawdust; Pine; Response surface methodology

Full Text:


Welcome to BioResources! This online, peer-reviewed journal is devoted to the science and engineering of biomaterials and chemicals from lignocellulosic sources for new end uses and new capabilities. The editors of BioResources would be very happy to assist you during the process of submitting or reviewing articles. Please note that logging in is required in order to submit or review articles. Martin A. Hubbe, (919) 513-3022, hubbe@ncsu.edu; Lucian A. Lucia, (919) 515-7707, lucia-bioresources@ncsu.edu URLs: bioresourcesjournal.com; http://ncsu.edu/bioresources ISSN: 1930-2126