Utilizing a Novel Fungal Enzymatic Cocktail as an Eco-Friendly Alternative for Cellulose Pulp Biobleaching

Vanessa Elisa Pinheiro, Jorge A. Ferreira, Jorge Henrique de Almeida Betini, Eliana Setsuko Kamimura, Maria de Lourdes Teixeira de Moraes Polizeli


Enzyme cocktails can alter the lignin and hemicellulose content in wood cell walls, improving the bleaching process during pulp production and offsetting the need for toxic chemicals. In this study, brown pulp was biobleached with a mixture of crude fungal extracts rich in xylanase and laccase, respectively produced from Aspergillus tamarii Kita and Trametes versicolor on waste materials. The optimal conditions for biobleaching were a mixture of xylanase and laccase crude extracts (1 to 2 v/v), at a temperature of 36 °C and a pH of 5.5. The treated brown cellulose pulp showed a reduction in the Kappa number by 1.83 points, representing an efficiency of 20.3%. In addition, the brightness increased by 4.65 points in comparison to the control. Hence, studies involving the application of the standardized cocktail during the hydrolysis of lignocellulosic residues, e.g., barley residue and sugarcane bagasse, led to the formation of 85 g/L and 25 g/L of reducing sugars, respectively. Moreover, the standardized cocktail caused greater deinking of the recycled paper pulp.


Enzymes; Aspergillus tamarii Kita; Trametes versicolor; Cellulose pulp; Biobleaching; Central composite design

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