The Transcriptome and Metabolic Pathways of Persea americana under Drought and Low-temperature Stress

Authors

  • Yi Zhang College of Forestry, Southwest Forestry University, Kunming, 650224, China; Laboratory of Forest Plant Cultivation and Utilization, The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Yunnan Academy of Forestry and Grassland, Kunming, 650201, China
  • Maniya Luo National Culture Research Institute of Jinggu Dai and Yi Autonomous County, Puer, 666400, China
  • Xiaolong Yuan Laboratory of Forest Plant Cultivation and Utilization, The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Yunnan Academy of Forestry and Grassland, Kunming, 650201, China
  • Zhijuan Yang College of Forestry, Southwest Forestry University, Kunming, 650224, China
  • Yi Wang Laboratory of Forest Plant Cultivation and Utilization, The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Yunnan Academy of Forestry and Grassland, Kunming, 650201, China
  • Yuan Zheng College of Forestry, Southwest Forestry University, Kunming, 650224, China

Keywords:

Persea americana, Drought stress, Low-temperature stress, Metabolic pathways, Transcriptome, qRT-PCR

Abstract

Persea americana Mill. is an important cash crop that contains effective ingredients to reduce cholesterol and protect the cardiovascular system. Presently, the gene regulation mechanism and signal pathway of stress response in P. americana are unclear. To explore the gene expression changes of P. americana under drought and low-temperature stress, the transcripts of P. americana were sequenced under these conditions. The results produced 42,815,960 bp raw reads. Analysis of the related metabolic pathways and differentially expressed genes showed that under drought stress, the gene expression of beta-amylase 3, glyceraldehyde-3-phosphate dehydrogenase and hexokinase were upregulated, while the gene expression of UDP-glycosyltransferase superfamily protein isoform, glucose-1-phosphate adenylyltransferase, and glucose-6-phosphate 1-epimerase were downregulated. Under low-temperature stress, the expression of beta-amylase and shikimate O-hydroxycinnamoyl transferase genes was downregulated. In addition, WRKY, MYB, bHLH, and NAC transcription factors were expressed under drought and low-temperature stress. Finally, the RNA-Seq data were validated using real-time fluorescence quantitative analysis to identify the key genes of P. americana regulated at the transcriptional level under drought and low-temperature stress. This study provides a theoretical basis for the selection of drought-resistant and low-temperature tolerant P. americana varieties. 

Downloads

Published

2023-04-20

Issue

Vol. 18 No. 2 (2023)

Section

Research Article or Brief Communication