Bioactive Metabolites from Germinated Cajanus cajan (L.) Millsp. Seeds after Treating with Different Concentrations of Salicylic Acid

Authors

  • Vinduja Vasudevan Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Aradhana Karthikeyan Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Tharun Prasanna Rajamuthu Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Ananya Sarkar Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Girinath Paranjothi Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Nagasathiya Krishnan Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Meenakumari Sakthivelu Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology
  • Palaniyandi Velusamy Research and Development Wing, Sree Balaji Medical College and Hospital (SBMCH), Bharath Institute of Higher Education and Research (BIHER)
  • Periasamy Anbu Department of Biological Engineering, Inha University
  • Pachaiappan Raman Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology

Keywords:

Salicylic acid, Cajanus cajan, Germination, Secondary metabolites, GC-MS

Abstract

Salicylic acid (SA) is an effective elicitor for enhancing product formation in various agricultural practices. This study examined the diverse responses to physiological metabolites and the pathway modifications in broad-spectrum resistance-1 (BSR1) of Cajanus cajan after treatment with SA using a metabolomics technique. The significance of the SA function at the metabolite level was examined by treating C. cajan with various concentrations of SA and germinated by soaking in water for different time periods. The secondary metabolites were recovered and investigated by gas chromatography-mass spectrometry for all the periodic conditions. Chemometric analysis of the collected samples showed that the seeds responded to the SA treatment. Acetic acid increased in germinated seeds after the SA treatment. In addition, the up-regulated metabolite production was downregulated in the C. cajan seeds before germination. The levels of metabolites, including hyacinthin, furaneol, citramalic acid, palmitate, stearate, linoleate, tocopherol, glucobrassicin, syringol, and hydroxy acetophenone, were increased after the SA treatment compared to control. Hence, the SA-treated seedling is a potential bio-factory for nutraceutical products to provide significant health benefits to the human population.

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Published

2023-04-25

Issue

Vol. 18 No. 2 (2023)

Section

Research Article or Brief Communication