A Comparative Assessment of Sea Buckthorn (Hippophae rhamnoides L.) Pruning Waste as a Potential Source of Serotonin

Authors

  • Sarmite Janceva Laboratory of Lignin Chemistry, Latvian State Institute of Wood Chemistry, Dzerbenes street 27, Riga, LV-1006 Latvia
  • Anna Andersone Laboratory of Lignin Chemistry, Latvian State Institute of Wood Chemistry, Dzerbenes street 27, Riga, LV-1006 Latvia; Ekokompozit Ltd, Dzerbenes street 27, Riga, LV-1006 Latvia
  • Liga Lauberte Laboratory of Finished Dosage Forms, Rīga Stradinš University, Dzirciema 16, LV-1007, Riga, Latvia
  • Natalija Zaharova Laboratory of Lignin Chemistry, Latvian State Institute of Wood Chemistry, Dzerbenes street 27, Riga, LV-1006 Latvia; Ekokompozit Ltd, Dzerbenes street 27, Riga, LV-1006 Latvia
  • Galina Telysheva Laboratory of Lignin Chemistry, Latvian State Institute of Wood Chemistry, Dzerbenes street 27, Riga, LV-1006 Latvia
  • Jelena Krasilnikova Riga Stradinš University, Department of Human Physiology and Biochemistry, Dzirciema 16, LV-1007 Riga, Latvia
  • Gints Rieksts Laboratory of Lignin Chemistry, Latvian State Institute of Wood Chemistry, Dzerbenes street 27, Riga, LV-1006 Latvia

Keywords:

Sea buckthorn, Serotonin, Twigs, Bark, Freon, Antimicrobial activity, Amylase activity

Abstract

Sea buckthorn (Hippophae rhamnoides L.) twigs, remaining after harvesting and pruning, are an underutilized and little-explored biomass resource. This study investigated the content of serotonin in 10 sea buckthorn cultivars (‘Maria Bruvele’, ‘Botanicheskaya Lubitelskaya’, ‘Tatiana’, ‘Otto’, ‘Leikora’, ‘Duet’, ‘Clara’, ‘Lord’, ‘Eva’, ‘Tarmo’) for the first time, and for further adjustment of the extraction conditions, cultivar ‘Maria Bruvele’ was extracted by water and water/ethanol solution with 20-25, 50, 70, and 96% ethanol at different temperatures. The results showed that 50% water/ethanol solutions are the most suitable for extraction, which makes it possible to increase the yield of serotonin. The 2-year-old twigs and bark from ‘Maria Bruvele’ collected in autumn contained higher serotonin content compared to spring-collected biomass. Serotonin sequential purification allowed the serotonin content in the fraction to increase to 26%/DM. The serotonin-rich fraction showed antimicrobial activity against gram-positive and gram-negative bacteria. In tests with salivary amylase, a serotonin-rich fraction at the amount of 0.1-0.4 mg/mL of saliva, under normal physiological conditions, tended to increase amylase activity, resulting in acceleration of starch degradation to glucose. Thus, the results support further study of the serotonin fraction for the treatment of people having underweight, malnutrition, and malabsorption conditions.

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Published

2023-12-12

Issue

Vol. 19 No. 1 (2024)

Section

Research Article or Brief Communication