Deciphering the Therapeutic, Larvicidal, and Chemical Pollutant Degrading Properties of Leaves-mediated Silver Nanoparticles Obtained from Alpinia purpurata

Authors

  • Manikandan Vani Raju Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
  • Meenakshi Kaniyur Chandrasekaran Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
  • Meenakshi Sundari Rajendran Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
  • Gopalakrishnan Velliyur Kanniappan Centre for Plant Tissue Culture and Central Instrumentation Facility, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
  • Rathi Muthaiyan Ahalliya Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
  • Guru Kumar Dugganaboyana Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research, Mysore, Karnataka, India
  • Mikhlid H Almutairi Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Riyadh, Saudi Arabia
  • Bader O. Almutairi Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Riyadh, Saudi Arabia
  • Ameer Khusro Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai – 600077, India
  • Ponnuswamy Vijayaraghavan Bioprocess Engineering Division, Smykon Biotech, Kanniyakumari, India

Keywords:

Alpinia purpurata, AgNPs, Cytotoxicity, Antimicrobial, Larvicidal, Photocatalytic activity

Abstract

The aim of the study was to synthesize silver nanoparticles (AgNPs) from Alpinia purpurata leaves and evaluate their cytotoxic, antimicrobial, antibiofilm, dye degradation, and larvicidal potentials. The synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, Fourier transform infrared, and high-resolution transmission electron microscopy, which confirmed AgNPs synthesis and revealed nanoparticle size (10 to 30 nm) and the presence of silver. Cytotoxicity tests showed IC50 values of 4.59 ± 0.6 µg/mL in A549 cells and 3.48 ± 0.4 µg/mL in PA1 cells, inducing apoptosis and DNA fragmentation. Flow cytometry revealed cell cycle arrest at G0-G1 phase. AgNPs exhibited significant antimicrobial activity, with maximum inhibition zones against K. pneumoniae (23 ± 2 mm) and F. oxysporum (17 ± 2 mm), and minimum inhibitory concentration (MIC) values ranging from 12.5 ± 0.25 to 75 ± 2.5 µg/mL. They also reduced bacterial and fungal biomass and showed antibiofilm effects. Photocatalytic activity degraded methylene blue dye by 88.4 ± 1.4% in 60 minutes. Larvicidal activity resulted in 100% mortality of A. aegypti larvae after 48 hours exposure to AgNPs (10 mg/L), additionally reducing chemical oxygen demand (55.1 ± 2.1% to 63.8 ± 1.5%) and microbial load in wastewater (2.5 to 10 ppm).

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Published

2024-04-12

Issue

Vol. 19 No. 2 (2024)

Section

Research Article or Brief Communication